Re: Ex Vivo Fluorescent Bone Stain?

Posted by Tim Feinstein on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Ex-Vivo-Fluorescent-Bone-Stain-tp7584365p7584367.html

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Agreed that SHG is the way to go.  Mineralized tissue is generally a real
pain for imaging.  

Is micro-CT out of the question?


Tim

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology





On 10/14/15, 1:30 PM, "Confocal Microscopy List on behalf of Steffen
Dietzel" <[hidden email] on behalf of
[hidden email]> wrote:

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>
>Jonathan,
>
>if you are using multi-photon anyway, have you tried second harmonic
>generation microscopy? Bone usually gives a decent signal and you do not
>even have to stain. Just hit the sample with twice the wavelength of
>whatever your favorite emission filter lets through.
>
>Steffen
>
>Am 14.10.2015 um 18:32 schrieb John Sevick:
>> *****
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>>posting.
>> *****
>>
>> Hello,
>>
>> I'm very new to microscopy, so I apologize in advance if this is the
>>wrong place to be posing
>> this question:
>>
>> I've been struggling to find a fluorescent bone mineral stain that
>>functions ex vivo. In the
>> literature it seems that most stains (calcein, alizarin complexone,
>>xylenol orange etc.) must
>> be injected in vivo to work properly ­ this is also what people in the
>>lab have been telling
>> me.
>>
>> Because I'm not familiar with how these stains work, I'm wondering if
>>there is a fluorescent
>> stain that can be applied to mineralized tissue ex vivo (not
>>necessarily cells, just the
>> mineralized matrix)?
>>
>> I am trying to differentiate mineralized tissue from soft tissue of
>>frozen, then thawed
>> samples using two-photon excitation microscopy.
>>
>> Any help would be greatly appreciated. Thank you!
>>
>> Johnathan Sevick, BASc| MSc Candidate
>> Biomedical Engineering Graduate Program | University of Calgary
>> Email: [hidden email]
>>
>>
>> --
>> ------------------------------------------------------------
>> Steffen Dietzel, PD Dr. rer. nat
>> Ludwig-Maximilians-Universität München
>> Biomedical Center (BMC)
>> Head of the Core Facility Bioimaging
>>
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