Posted by
Anil Poudel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/disinfecting-a-microscope-tp7584530p7584532.html
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Hello there,
I am new to fluorescence microscopy and trying some F-actin staining kit (Phalloidin 555 from Invitrogen) but having hard time to get very clean filamentous picture and also unable to get the image on screen as it appears through eyepiece. I am using Olympus IX71 with qclick monochorome camera and cellsens software. Any suggestion would for optimization is highly appreciated. Thanks in advance.
Anil
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From: Confocal Microscopy List <
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Sent: Wednesday, December 16, 2015 2:01 PM
To:
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Subject: disinfecting a microscope
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What is an appropriate method for disinfecting a microscope used for live cell imaging (or in a BSL2 facility)? 10% bleach is too strong, so what do people use? Thinking of core facility users (who can be poor at following directions), what is a relatively fool-proof way to decontaminate and not damage motorized stages, and other delicate parts?
If you have posted protocols, can you send the links? Do you have live cell imaging policies for your facility that you would be willing to share?
Doug
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Douglas W. Cromey, M.S. - Associate Scientific Investigator
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