Posted by
Sathya on
Dec 17, 2015; 5:45pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/disinfecting-a-microscope-tp7584530p7584540.html
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Hi Doug,
I was recently discussing about this issue to our Biosafety officers. What they say is: "Appropriate disinfection will depend on the nature of agent or the biohazard. A risk assessment will have to performed by the biosafety officer to determine appropriate disinfectant on a case by case basis. Assuming that all live samples will be sealed so the microscope technically should not come in contact with biohazardous material. So in general 70% Ethanol can be used as a general disinfectant. But, may have to consider other disinfectants (based on risk and agents) that are not corrosive to the microscope optics".
I was digging more on it on the Biosafety manual of the institute and came across - Quaternary ammonium compounds which are not recommended for spill clean-up, but may be utilized for surface decontamination. Manufacturer’s claims for newer formulations, like Coverage Plus NPD (
http://www.steris.com/products/cleaners-and-disinfectants/coverage-plus-npd) and Roccal D-Plus (
https://www.zoetisus.com/products/cats/roccal-d-plus.aspx), indicate that these are effective against most bacteria, viruses, and some fungi. They are relatively non-corrosive, so may be useful for surface disinfection of sensitive instruments. One thing that I am seeing that both Coverage Plus NPD and Roccal D-Plus comes with deodorant. No commercial interest. Good luck.
Sathya Srinivasan
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Subject: Re: disinfecting a microscope
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Thank you Dr. Periasamy. Are there any others? Does anyone have a BSL2 protocol already written up for a microscope core facility? I'd like not to re-invent the wheel and some of you have probably had to do this already.
Thanks.
Doug
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Douglas W. Cromey, M.S. - Associate Scientific Investigator
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Sent: Wednesday, December 16, 2015 12:13 PM
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Subject: Re: disinfecting a microscope
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Doug
This is what we use approved by our University Biosafety "Cavi Cide" you can spray on the microscopy stage and the desk area and wipe it.
Hope this helps.
Ammasi
Dr. Ammasi Periasamy
Professor & Center Director
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What is an appropriate method for disinfecting a microscope used for live cell imaging (or in a BSL2 facility)? 10% bleach is too strong, so what do people use? Thinking of core facility users (who can be poor at following directions), what is a relatively fool-proof way to decontaminate and not damage motorized stages, and other delicate parts?
If you have posted protocols, can you send the links? Do you have live cell imaging policies for your facility that you would be willing to share?
Doug
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Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
office: UACC 0914A email:
[hidden email]<mailto:
[hidden email]>
voice: 520-626-2824 fax: 520-626-2097
http://swehsc.pharmacy.arizona.edu/microHome of: "Microscopy and Imaging Resources on the WWW"
UA Microscopy Alliance -
http://microscopy.arizona.edu<
http://microscopy.arizona.edu/>