Re: Exporting 8 bits color .tif images in Cell Sens

Posted by Ondřej Šebesta on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Exporting-8-bits-color-tif-images-in-Cell-Sens-tp7584524p7584550.html

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Hello Juan

Despite you are absolutely right, that Olympus should improve export
capabilities of IMHO any of their software, ImageJ is surely able to
convert many formats. I do not have personal experience with .vsi files,
but I guess Bioformats would import correctly and resave is easy. You can
use a macro recorder to get a primitive macro which can do the job for you.
Batch process - macro is another usefull tool.

Best, Ondrej Sebesta

2015-12-16 14:42 GMT+01:00 Juan Luis Ribas <[hidden email]>:

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>
> Thank you for all the people that reply, but unfortunately I have to say
> that at the end all the procedures finished in gray scale images .tif
> images.
> Definitevely, it should be named CellNoSens or Cell-nonSens. I wonder if
> the vendor could argue something about this question. About 50 users in my
> facility moved to another microscope beside. The companies has to design
> the software to do the life easier...
> On the other hand, perhaps some expert in ImageJ could know if there is a
> plugin in ImageJ that could convert .vsi files to .tif 8 bit color .
>
> Best regards
> Juan Luis Ribas
>
>
> El 16/12/2015 a las 2:09, Sathya Srinivasan escribió:
>
>> Hi Juan,
>> Have you tried the following in CellSens:
>>
>> select Image>Mode>8 bit/Channel menu command to change the image into
>> 8‐bit
>> image. Use File>Save as… menu command to save the image as one image
>> containing multiple channels or use
>> File>Export to>tiff series… command to save the image as multiple images
>> for each channel
>>
>> I do not have access to CellSens right now. I had this notes which I have
>> collected from some web site with me and thought of sharing it with you.
>> Good luck.
>>
>> Sathya Srinivasan
>>
>> ________________________________________
>> From: Confocal Microscopy List <[hidden email]> on
>> behalf of Juan Luis Ribas <[hidden email]>
>> Sent: December 15, 2015 7:09 AM
>> To: [hidden email]
>> Subject: Exporting 8 bits color .tif images in Cell Sens
>>
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>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>>
>> Dear Coleagues,
>> Last year we upgraded our BX61 with CellSens software and a new DP73
>> camera. Almost all of our users wants to get multichannel 8 bits color
>> images in ".tif" format and the software cannot get them without
>> exporting each channel separately, which is very time consuming.
>> Multichannel acquisition does not work in 8 bits mode. In my opinion it
>> should be a decision of the user to work with 8 or 16 bits acquisition
>> and also with gray or color images.
>> As an alternative, I've tried to configure a 8 bits multichannel color
>> acquisition with Experiment Manager but the system produce 3 separated
>> .vsi images for each channel and you have to go one by one converting
>> ".vsi" to ".tif" format.
>> I wonder if someone has an alternative way to acquire quickly (one mouse
>> click) multichannel 8 bit color images and generate ".tif" images for
>> each channel?
>>
>> Thank you very much in advance.
>>
>> All the best
>> Juan Luis Ribas
>> --
>> Juan Luis Ribas
>> Servicio de Microscopía
>> Centro de Investigación, Tecnología e Innovación
>> Universidad de Sevilla
>> Av. Reina Mercedes 4b
>> 41012 Sevilla
>>
>> Tfnos: 954559983 // 628220681 // 12068
>>
>> http://investigacion.us.es/scisi/sgi/servicios/microscopia/equipamiento
>>
>
> --
> Juan Luis Ribas
> Servicio de Microscopía
> Centro de Investigación, Tecnología e Innovación
> Universidad de Sevilla
> Av. Reina Mercedes 4b
> 41012 Sevilla
>
> Tfnos: 954559983 // 628220681 // 12068
>
> http://investigacion.us.es/scisi/sgi/servicios/microscopia/equipamiento
>
> --
> <http://investigacion.us.es/scisi/sgi/servicios/microscopia/equipamiento>
> Mgr. Ondřej Šebesta
> E-mail:
> <http://investigacion.us.es/scisi/sgi/servicios/microscopia/equipamiento>
> [hidden email]
>
> Univerzita Karlova v Praze
> Přírodovědecká fakulta
> Albertov 6, 128 43 Praha 2
> www.natur.cuni.cz
>
> Charles University in Prague
> Faculty of Science
> Albertov 6, 128 43 Praha 2
> www.natur.cuni.cz/en
>
>

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