Re: Dichroic mirror double reflections issue

Posted by Lu Yan on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Dichroic-mirror-double-reflections-issue-tp7584577p7584582.html

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Hi Sripad,

I am not sure if it has the AR coating on the other side. But I am now
getting help from the vendor. Hopefully it will solved soon. I am using it
with 45 degree incidence.

Happy New Year!

Lu

-----------------------------------------------------
Lu Yan
Nanostructured Fibers and Nonlinear Optics Laboratory
Electrical and Computer Engineering
Boston University
8 St. Mary St., Boston, MA, 02215
617.353.0286 (office) | 617.358.5917 (lab)
[hidden email]
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On Wed, Dec 30, 2015 at 6:04 PM, Sripad Ram <[hidden email]> wrote:

> *****
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> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Yan,
> The issue sounds like the dichroic does not have an anti-reflection coating
> on the non-reflective side. From the URL it was not clear if the optic has
> the AR coating. Also are you using the dichroic at 45 degree incidence.
>
> HTH
>
> Sripad
>
> On Wed, Dec 30, 2015 at 10:05 AM, Yan, Lu <[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi there,
> >
> > I recently bought a dichroic mirror from Chroma (2mm thick reflecting
> > fluorescence,
> > https://www.chroma.com/products/custom-inventory/nc302987-zt670dcrb) for
> > our confocal fluorescence microscopy setup. But I found that with the
> > reflective side oriented correctly, at the reflection band, the reflected
> > beams seemed splitting into two and partially overlapping with each other
> > (ghost image?). This happens at a wide range of incident angles, and as I
> > rotated the dichroic (changing the angle), the "overlapped" reflection
> > alternated between partially overlapping and two distinct separated (by a
> > dark line in the middle) half circular beams.
> >
> > Images of single fluorescence bead also showed 'side lobes'. So I was
> > wondering if anyone has experience on how to eliminate such effect? Is
> this
> > common for dichroics? Or it is specific for the one I have and I should
> get
> > another one?
> >
> > Thank you very much in advance, and I wish you all Happy New Year!
> >
> > Lu
> > -----------------------------------------------------
> > Lu Yan
> > Nanostructured Fibers and Nonlinear Optics Laboratory
> > Electrical and Computer Engineering
> > Boston University
> > 8 St. Mary St., Boston, MA, 02215
> > 617.353.0286 (office) | 617.358.5917 (lab)
> > [hidden email]
> > -----------------------------------------------------
> >
>