Re: Basic question

Posted by Julio Vazquez-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Basic-question-tp7584586p7584587.html

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There is a formula on page 11 of a publication by Zeiss titled "Confocal Laser Scanning Microscopy Principles" for calculating the thickness of confocal optical sections:

http://zeiss-campus.magnet.fsu.edu/referencelibrary/pdfs/ZeissConfocalPrinciples.pdf


best,

Julio Vazquez
Fred Hutchinson Cancer Research Center

----- Original Message -----
From: "Christian Elowsky" <[hidden email]>
To: "Confocal Microscopy List" <[hidden email]>
Sent: Monday, January 4, 2016 12:36:40 PM
Subject: Basic question

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I have a basic question, how does one calculate the physical plane from a
confocal image?  Meaning without under or oversaturation, what is the plane
thickness calculation if the pinhole is set to 1 A.U.?

Basically, I have a 4x image z-series from a Nikon A1 and need to explain
what physically is represented in each plane.  The pinhole was set to 1 A.U.

Unless of course the calculations are in the image data and I've had no luck
finding it yet.  I'm also happy to figure it out, if someone wants to point
me at the right textbook.

Thanks.
--
Julio Vazquez, PhD
Director of Scientific Imaging
Office: 206.667.1215
Lab: 206.667.4205
[hidden email]


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