Posted by
Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Resonant-scanning-preamplifer-bandwidth-tp7584591p7584592.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
Hi Heping, the analog bandwidth of the amplifier will smear out fast photon
events if it is too slow. This can lead to sampling errors if a single
photon event gets spread across multiple digital sampling windows when
presented to the A/D.
Craig
On Jan 4, 2016 10:16 PM, "Heping Yuan" <
[hidden email]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Hello list users. I had a question about analog to digital conversion in
> resonant scanning two
> photon microscopes. I have seen older discussions involving some users of
> the open source
> ScanBox and ScanImage programs and they advocate for using sampling rates
> that match
> with the Ti-Sapphire laser repetition rate (80 MHz). However, they also
> suggest using
> preamplifiers with cutoffs that match this sampling rate (80 MHz). Should
> the preamplifier
> not be at most half of the sampling rate to avoid aliasing? I believe
> there may be more
> subtle details I am not aware of.
>
> Thank you for your time.
>
> Heping
>