Posted by
George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Resonant-scanning-preamplifer-bandwidth-tp7584591p7584612.html
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DRAQ7
Manufacturer web page is
http://www.biostatus.com/DRAQ7In the US available from several distributors ... Google DRAQ7.
Plan H, N or NPC: you could instead use an Cy5.5 etc anti-histone. Even
better: anti-Lamin, just label the nuclear envelope; if you are a
pointillist, anti-nuclear pore complex protein, see
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916659/figure/F3/Less is more: the fewer pixels or voxels you obscure with counterlabel,
the cleaner your background and higher signal to background. To coin a
phrase" don't waste voxels on counterstains".
For paraformaldehyde fixed cells, I see nice cytoplasmic structures
(maybe mitochondria) prominently in the green channel, also most longer
channels.
On 1/8/2016 9:14 AM, Vitaly Boyko wrote:
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> Dear All,
> I am looking for a nuclear dye that is on the other side of the spectra, 700-900 nm emission range (as we are planing to use ATTO 390 for immunostainings instead of dapi, Hoechst). Any suggestions/guidance will be appreciated. TO-PRO-5 is no longer available.
> Thank you.
> Vitaly
>
--
George McNamara, Ph.D.
Single Cells Analyst, T-Cell Therapy Lab (Cooper Lab)
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales
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