Posted by
Stanislav Vitha-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/reflected-light-profilometry-trrough-plastic-mystery-bands-tp7584686p7584688.html
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Craig,
the scattering is not that much.
The glue is applied to the spacer only, so there should be no glue over the
clear channel.
The Mylar top and bottom sheets look optically clear.
The spacer is also mylar but is opaque/black.
Perhaps it has something to do with birefringence of the plastics, but I did
not quite think it through.
On Fri, 29 Jan 2016 14:02:31 -0700, Craig Brideau
<
[hidden email]> wrote:
>Looks like a lot of scatter off something in the sample. When you scan and
>look at the sample sitting on the stage do you see a lot of light off the
>sample? I mean by eyeball, not with the microscope optics. It's possible
>your glue is scattering and confusing your interfaces, also possible that
>the mylar is very scattering. You can also have lots of multiple
>reflections between surfaces, although the intensity of such 'ghost'
>reflections tend to be much lower than the main reflection. Finally, what
>size confocal pinhole are you using, and have you tried other
wavelengths?
>
>Craig Brideau
With regards,
>>
>> Stan Vitha
>> Microcopy and Imaging Cneter
>> Texas A&M University
>>