Re: reflected light profilometry trrough plastic - mystery bands

Posted by Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/reflected-light-profilometry-trrough-plastic-mystery-bands-tp7584686p7584694.html

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 From your image it seems much easier to estimate the thickness of the
spacer rather than the one of the air filled chamber. Not surprising
considering the mess in optical properties you have in the air chamber
light path in combination with the high NA air objective (see Guy's
post). Is spacer thickness not good enough?

Also, if the low NA objective approach Guy suggested should not work
out, maybe you can align the mylar with the polarization of the laser
beam - what happens if you turn the sample?

Steffen

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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de