Posted by
Stanislav Vitha-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/reflected-light-profilometry-trrough-plastic-mystery-bands-tp7584686p7584700.html
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Hi Steffen,
the spacer thickness would probably be good enough but the spacer is very
opaque (black) and the reflections not very strong, given that it is the
same plastic with just a thin layer of glue holding it together. That is why I
went initially for the brighter signal in the air channel.
I will try the 10x/0.4 objective and also try rotating the sample.
Stan Vitha
Microscopy and Imaging Center
Texas A&M University
so the signal was
On Mon, 1 Feb 2016 14:49:38 +0100, Steffen Dietzel
<
[hidden email]> wrote:
> From your image it seems much easier to estimate the thickness of the
>spacer rather than the one of the air filled chamber. Not surprising
>considering the mess in optical properties you have in the air chamber
>light path in combination with the high NA air objective (see Guy's
>post). Is spacer thickness not good enough?
>
>Also, if the low NA objective approach Guy suggested should not work
>out, maybe you can align the mylar with the polarization of the laser
>beam - what happens if you turn the sample?
>
>Steffen
>
>--
>------------------------------------------------------------
>Steffen Dietzel, PD Dr. rer. nat
>Ludwig-Maximilians-Universität München
>Biomedical Center (BMC)
>Head of the Core Facility Bioimaging
>
>Großhaderner Straße 9
>D-82152 Planegg-Martinsried
>Germany
>
>
http://www.bioimaging.bmc.med.uni-muenchen.de