Clearing reagents with water dipping objective

Posted by Moulding, Dale on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Clearing-reagents-with-water-dipping-objective-tp7584708.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear List,
I am looking for some advice regarding using a water dipping objective without a coverslip
and immersing directly in clearing solutions.
We have a Zeiss 20x dipper NA1.0 with correction collar (421452-9700-000).
Is this suitable to dip directly into Scalea2 and its derivatives? Is there any risk of damaging
the objective?
Cheers
Dale