Re: Clearing reagents with water dipping objective

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Clearing-reagents-with-water-dipping-objective-tp7584708p7584713.html

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Most of the clearing solutions/index-matching liquids will wreck any
objective they come in contact with. The solvents used in these are pretty
nasty.
The only exceptions I can think of are the sugar-based techniques such as
SeeDB. These use fructose etc for the index-matching solution so they don't
dissolve the cement holding your lens together!

Craig

On Mon, Feb 8, 2016 at 2:36 AM, Dale Moulding <[hidden email]> wrote:

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> Dear List,
> I am looking for some advice regarding using a water dipping objective
> without a coverslip
> and immersing directly in clearing solutions.
> We have a Zeiss 20x dipper NA1.0 with correction collar (421452-9700-000).
> Is this suitable to dip directly into Scalea2 and its derivatives? Is
> there any risk of damaging
> the objective?
> Cheers
> Dale
>