Re: Clearing reagents with water dipping objective

Posted by Zdenek Svindrych-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Clearing-reagents-with-water-dipping-objective-tp7584708p7584714.html

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We've been told that water-based solutions are generally safe to use with
dipping lenses (I would not be afraid to use clearing solutions based on
glycerol, TDE, urea, histodenz, DMSO, fructose, focus clear, ...). Formamide
may corrode the metal parts of the lens and organic solvents (BABB, DBE,
THF, Methyl salicylate ...) may dissolve the lens cement.
Best, zdenek

--
Zdenek Svindrych, Ph.D.
W.M. Keck Center for Cellular Imaging (PLSB 003)
University of Virginia, Charlottesville, VA
http://www.kcci.virginia.edu/
tel: [phone]


---------- Původní zpráva ----------
Od: Craig Brideau <[hidden email]>
Komu: [hidden email]
Datum: 8. 2. 2016 17:29:40
Předmět: Re: Clearing reagents with water dipping objective

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Most of the clearing solutions/index-matching liquids will wreck any
objective they come in contact with. The solvents used in these are pretty
nasty.
The only exceptions I can think of are the sugar-based techniques such as
SeeDB. These use fructose etc for the index-matching solution so they don't
dissolve the cement holding your lens together!

Craig

On Mon, Feb 8, 2016 at 2:36 AM, Dale Moulding <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear List,
> I am looking for some advice regarding using a water dipping objective
> without a coverslip
> and immersing directly in clearing solutions.
> We have a Zeiss 20x dipper NA1.0 with correction collar (421452-9700-000).
> Is this suitable to dip directly into Scalea2 and its derivatives? Is
> there any risk of damaging
> the objective?
> Cheers
> Dale
>"