Re: Clearing reagents with water dipping objective

Posted by Davide Accardi on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Clearing-reagents-with-water-dipping-objective-tp7584708p7584715.html

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Dear Dale,

I would like to make a note on the lens you are planning to use.
Th ZEISS 20x/1.0 is an optimised water dipping lens with a correction collar that allows to compensate for variation of the dipping solution refractive index in a range that spans from 1.30 to 1.36. Therefore, consider that making the experiment you briefly described you will have signifiant image aberrations due to the refractive index mismatch between the clearing solution and what the lens is optimised for.

Good luck

Davide
--
Dr. Davide Accardi
Light Microscopy Facility
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany

Phone: [phone]

> On 08 Feb 2016, at 10:36, Dale Moulding <[hidden email]> wrote:
>
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> Dear List,
> I am looking for some advice regarding using a water dipping objective without a coverslip
> and immersing directly in clearing solutions.
> We have a Zeiss 20x dipper NA1.0 with correction collar (421452-9700-000).
> Is this suitable to dip directly into Scalea2 and its derivatives? Is there any risk of damaging
> the objective?
> Cheers
> Dale