Re: Clearing reagents with water dipping objective

Posted by Tim Feinstein on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Clearing-reagents-with-water-dipping-objective-tp7584708p7584717.html

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As I recall Olympus makes a couple of super-objectives designed for
ScaleView-A2, iDISCO etc, 10x and 25x I believe.  The other manufacturers
should have something comparable by now.  None of them will be cheap.

Best,


Tim

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology





On 2/8/16, 4:36 AM, "Confocal Microscopy List on behalf of Dale Moulding"
<[hidden email] on behalf of [hidden email]> wrote:

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>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
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>
>Dear List,
>I am looking for some advice regarding using a water dipping objective
>without a coverslip
>and immersing directly in clearing solutions.
>We have a Zeiss 20x dipper NA1.0 with correction collar
>(421452-9700-000).
>Is this suitable to dip directly into Scalea2 and its derivatives? Is
>there any risk of damaging
>the objective?
>Cheers
>Dale