Re: Clearing reagents with water dipping objective

Posted by Moulding, Dale on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Clearing-reagents-with-water-dipping-objective-tp7584708p7584720.html

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Dear all,

many thanks for the helpful replies. I also heard back from my local Zeiss team, and they
say the water based reagents won't harm the objective, as long as it is cleaned with
plenty of water to prevent crystals forming after use. As Davide and Zeiss pointed out,
the RI of the objective is not ideal, but to quote from Zeiss "it's worth a try". Surely using
an objective with correction to 1.36 (for the 1.38 Scalea2) is better than a dry objective?
The correction collar has gradations past the 1.36 mark, I wonder if that gives further
correction?
There are of course specialised objectives we could use, but they are indeed very
expensive, so as a first try we will give the water dipper a go.

cheers

Dale

On Tue, 9 Feb 2016 13:40:22 +0000, Feinstein, Timothy N <[hidden email]> wrote:

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>
>As I recall Olympus makes a couple of super-objectives designed for
>ScaleView-A2, iDISCO etc, 10x and 25x I believe.  The other manufacturers
>should have something comparable by now.  None of them will be cheap.
>
>Best,
>
>
>Tim
>
>Timothy Feinstein, Ph.D.
>Research Scientist
>University of Pittsburgh Department of Developmental Biology
>
>
>
>
>
>On 2/8/16, 4:36 AM, "Confocal Microscopy List on behalf of Dale Moulding"
><[hidden email] on behalf of [hidden email]>

wrote:

>
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>>Post images on http://www.imgur.com and include the link in your posting.
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>>
>>Dear List,
>>I am looking for some advice regarding using a water dipping objective
>>without a coverslip
>>and immersing directly in clearing solutions.
>>We have a Zeiss 20x dipper NA1.0 with correction collar
>>(421452-9700-000).
>>Is this suitable to dip directly into Scalea2 and its derivatives? Is
>>there any risk of damaging
>>the objective?
>>Cheers
>>Dale