Re: Standards for SIM resolution

Posted by Indig, Fred (NIH/NIA/IRP) [E] on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Standards-for-SIM-resolution-tp7584830p7584832.html

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Alison, how is this better than sub-resolution beads?

Sincerely,
Fred


Fred E. Indig, Ph.D.
Head, Confocal Imaging Facility

Biomedical Research Center (BRC) Rm 8B135
National Institute on Aging/NIH
251 Bayview Blvd.
Baltimore, MD  21224-6825

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________________________________________
From: Alison North [[hidden email]]
Sent: Tuesday, March 01, 2016 3:39 PM
To: [hidden email]
Subject: Re: Standards for SIM resolution

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Hi Josh,

We have used both the SIM nanorulers to check our OMX and the 80RG PAINT
nanorulers to check our STORM system.  Both work well though they can be
a bit tricky to find at first.  We have had less luck yet with the STORM
nanorulers in solution, so I would be interested to hear if anybody has
worked out the kinks for that sample!
Give me a call if you want to talk about it,

Best,
Alison

On 3/1/2016 2:42 PM, Joshua Zachary Rappoport wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Does anyone have a good recommended standard for SIM resolution?
> Has anyone tried these?
> http://www.gattaquant.com/products/gatta-sim-nanoruler.html
>
> Thanks!
>
> Josh