Posted by
Sylvie Le Guyader on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Standards-for-SIM-resolution-tp7584830p7584834.html
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Hi Alison
Just out of curiosity, why did you choose the 80RG and not the 20nm one since you wanted to calibrate STORM? Or was the 80 was only your best second choice since the STORM nanorulers did not work?
Med vänlig hälsning / Best regards
Sylvie
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Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7,
Novum, G lift, floor 6
14157 Huddinge
Sweden
mobile: +46 (0) 73 733 5008
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LCI website
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Alison North
Sent: den 1 mars 2016 21:39
To:
[hidden email]
Subject: Re: Standards for SIM resolution
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Hi Josh,
We have used both the SIM nanorulers to check our OMX and the 80RG PAINT nanorulers to check our STORM system. Both work well though they can be a bit tricky to find at first. We have had less luck yet with the STORM nanorulers in solution, so I would be interested to hear if anybody has worked out the kinks for that sample!
Give me a call if you want to talk about it,
Best,
Alison
On 3/1/2016 2:42 PM, Joshua Zachary Rappoport wrote: