http://confocal-microscopy-list.275.s1.nabble.com/Oil-objective-maximum-area-distance-for-long-term-timelapse-imaging-tp7584835p7584838.html
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>
> We regularly image the 4 center wells (about 50 total points) of a Labtek 8
> well chambered coverslip for 8-16 hours at 37c using PFS on a TiE.
>
> First we put the plate on, one drop of oil on the 60X or 100X objective,
> and drive to all four wells.
>
> Then we take the plate off, and add one more drop of oil, and we're good to
> go.
>
> We also slow the stage speed way down so PFS can follow the glass.
>
> Hope this helps.
>
> Gary
>
> On Wed, Mar 2, 2016 at 7:49 AM, Sylvie Le Guyader <
[hidden email]
> >
> wrote:
>
> > Hi Ken
> >
> >
> >
> > My experience: imaging 2 cells 1.7mm apart (thickness of the well wall in
> > the MW plates we were using then) every 10 min for 12h with a 60x oil
> > objective and a hardware autofocus at 37 deg resulted in a high risk
> (about
> > 25% of the cases?) of losing focus. There go years of sweat and tears…
> >
> >
> >
> > We were lucky to get to test a super device manufactured by EMBLEM, the
> > commercial side of EMBL. You send your objective. They custom make a cap
> > and send you a kit containing cap, tubes, pump and instructions. It is a
> > bit clumsy because you need to remove all other objectives and you need
> to
> > be careful not to put oil all over the place but it works a charm. I have
> > imaged 10 positions in each well over 15 wells every 10 min for 15 h with
> > this device without losing focus. :) You could contact them<
> >
http://www.embl-em.de/articles.php?lang=de&hid=4&Cat=Technology_Offers>
> > to ask if they commercialize it.
> >
> >
> >
> > Med vänlig hälsning / Best regards
> >
> >
> >
> > Sylvie
> >
> >
> >
> > @@@@@@@@@@@@@@@@@@@@@@@@
> >
> > Sylvie Le Guyader, PhD
> >
> > Live Cell Imaging Facility Manager
> >
> > Karolinska Institutet- Bionut Dpt
> >
> > Hälsovägen 7,
> >
> > Novum, G lift, floor 6
> >
> > 14157 Huddinge
> >
> > Sweden
> >
> > mobile: +46 (0) 73 733 5008
> >
> > office: +46 (0) 08-524 811 72 New number!
> >
> > LCI website
> >
> >
> >
> >
> >
> > -----Original Message-----
> > From: Confocal Microscopy List [mailto:
[hidden email]]
> > On Behalf Of Kenneth Chen
> > Sent: den 2 mars 2016 13:22
> > To:
[hidden email]
> > Subject: Oil objective maximum area/distance for long-term timelapse
> > imaging
> >
> >
> >
> > *****
> >
> > To join, leave or search the confocal microscopy listserv, go to:
> >
> >
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> > Post images on
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> posting.
> >
> > *****
> >
> >
> >
> > Hi all,
> >
> >
> >
> > We're in the process of designing microfluidic devices for multi-day
> > time-lapse imaging. In the past we've had some trouble with image quality
> > for very large devices deteriorating due to the loss/thinness of the
> > immersion oil layer between sample and objective when very large areas
> are
> > scanned. We have only used traditional oil objectives (no water or
> >
> > glycerol) Does anyone have any strategies to mitigate this or rules of
> > thumb about "safe" maximum sampling areas or distances to travel?
> >
> >
> >
> > Thanks,
> >
> > Ken Chen
> >
>
>
>
> --
> Best,
>
> Gary Laevsky, Ph.D.
> Confocal Imaging Facility Manager
> Dept. of Molecular Biology
> Washington Rd.
> Princeton University
> Princeton, New Jersey, 08544-1014
> (O) 609 258 5432
> (C) 508 507 1310
>