Piezo scanning stage induced image distortion

Posted by Lu Yan on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Piezo-scanning-stage-induced-image-distortion-tp7584896.html

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Dear listers,

I am encountering a problem with my piezo scanning stage (sample scanning)
in the microscopy setup. I found that at some discrete line scanning
frequencies (lines/s), the grating sample I imaged got some distortions
along the fast axis (the horizontal axis in following images). See this
link for some images:
https://www.dropbox.com/s/9r0e2rea8oaepgi/Screenshot%20-%203_17_2016%20%2C%209_49_00%20AM.png?dl=0

As you can see, for example, at 30 Hz, it seemed that the grating was
splitting into multiple lines; and at 40Hz, they are also pretty bad. I am
using a bidirectional sawtooth voltage pattern to feed the piezo. So I was
wondering if anyone has similar experience on this? I switched to a new
scanning stage, but the distortion still existed, and it did not change as
field of view and pixel size. Does anyone have an ideal what might be wrong?

Thanks a lot,
Lu