http://confocal-microscopy-list.275.s1.nabble.com/Piezo-scanning-stage-induced-image-distortion-tp7584896p7584897.html
> On Mar 17, 2016, at 10:08 AM, Yan, Lu <
[hidden email]> wrote:
>
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> Dear listers,
>
> I am encountering a problem with my piezo scanning stage (sample scanning)
> in the microscopy setup. I found that at some discrete line scanning
> frequencies (lines/s), the grating sample I imaged got some distortions
> along the fast axis (the horizontal axis in following images). See this
> link for some images:
>
https://www.dropbox.com/s/9r0e2rea8oaepgi/Screenshot%20-%203_17_2016%20%2C%209_49_00%20AM.png?dl=0>
> As you can see, for example, at 30 Hz, it seemed that the grating was
> splitting into multiple lines; and at 40Hz, they are also pretty bad. I am
> using a bidirectional sawtooth voltage pattern to feed the piezo. So I was
> wondering if anyone has similar experience on this? I switched to a new
> scanning stage, but the distortion still existed, and it did not change as
> field of view and pixel size. Does anyone have an ideal what might be wrong?
>
> Thanks a lot,
> Lu