Re: Piezo scanning stage induced image distortion

Posted by Lu Yan on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Piezo-scanning-stage-induced-image-distortion-tp7584896p7584898.html

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Hi Eric,

The images are all taken at the fixed sample plane. The sample is a simple
grating pattern. I took these images at different scanning speed.

Lu

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Lu Yan
Nanostructured Fibers and Nonlinear Optics Laboratory
Electrical and Computer Engineering
Boston University
Rm. 505, 8 Saint Mary's St., Boston, MA 02215
+1.617.353.0286 (office) | 617.358.5917 (lab)
[hidden email]
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On Thu, Mar 17, 2016 at 10:37 AM, Eric Marino <[hidden email]> wrote:

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> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Lu,
>
> Are the images a single plane or projected images?
>
> Eric Marino
> [hidden email]
>
>
>
> > On Mar 17, 2016, at 10:08 AM, Yan, Lu <[hidden email]> wrote:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear listers,
> >
> > I am encountering a problem with my piezo scanning stage (sample
> scanning)
> > in the microscopy setup. I found that at some discrete line scanning
> > frequencies (lines/s), the grating sample I imaged got some distortions
> > along the fast axis (the horizontal axis in following images). See this
> > link for some images:
> >
> https://www.dropbox.com/s/9r0e2rea8oaepgi/Screenshot%20-%203_17_2016%20%2C%209_49_00%20AM.png?dl=0
> >
> > As you can see, for example, at 30 Hz, it seemed that the grating was
> > splitting into multiple lines; and at 40Hz, they are also pretty bad. I
> am
> > using a bidirectional sawtooth voltage pattern to feed the piezo. So I
> was
> > wondering if anyone has similar experience on this? I switched to a new
> > scanning stage, but the distortion still existed, and it did not change
> as
> > field of view and pixel size. Does anyone have an ideal what might be
> wrong?
> >
> > Thanks a lot,
> > Lu
>