Re: Piezo scanning stage induced image distortion

Posted by Eric Marino on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Piezo-scanning-stage-induced-image-distortion-tp7584896p7584899.html

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Have you looked at the ramp voltage on an oscilloscope to check for noise? What camera are you using (sCMOS, CCD)? are you decreasing the exposure when you increase the scanning frequency?


Eric Marino
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> On Mar 17, 2016, at 10:59 AM, Yan, Lu <[hidden email]> wrote:
>
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>
> Hi Eric,
>
> The images are all taken at the fixed sample plane. The sample is a simple
> grating pattern. I took these images at different scanning speed.
>
> Lu
>
> ------------------------------------------------------------------
> -------------
> Lu Yan
> Nanostructured Fibers and Nonlinear Optics Laboratory
> Electrical and Computer Engineering
> Boston University
> Rm. 505, 8 Saint Mary's St., Boston, MA 02215
> +1.617.353.0286 (office) | 617.358.5917 (lab)
> [hidden email]
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> On Thu, Mar 17, 2016 at 10:37 AM, Eric Marino <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hi Lu,
>>
>> Are the images a single plane or projected images?
>>
>> Eric Marino
>> [hidden email]
>>
>>
>>
>>> On Mar 17, 2016, at 10:08 AM, Yan, Lu <[hidden email]> wrote:
>>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> Post images on http://www.imgur.com and include the link in your
>> posting.
>>> *****
>>>
>>> Dear listers,
>>>
>>> I am encountering a problem with my piezo scanning stage (sample
>> scanning)
>>> in the microscopy setup. I found that at some discrete line scanning
>>> frequencies (lines/s), the grating sample I imaged got some distortions
>>> along the fast axis (the horizontal axis in following images). See this
>>> link for some images:
>>>
>> https://www.dropbox.com/s/9r0e2rea8oaepgi/Screenshot%20-%203_17_2016%20%2C%209_49_00%20AM.png?dl=0
>>>
>>> As you can see, for example, at 30 Hz, it seemed that the grating was
>>> splitting into multiple lines; and at 40Hz, they are also pretty bad. I
>> am
>>> using a bidirectional sawtooth voltage pattern to feed the piezo. So I
>> was
>>> wondering if anyone has similar experience on this? I switched to a new
>>> scanning stage, but the distortion still existed, and it did not change
>> as
>>> field of view and pixel size. Does anyone have an ideal what might be
>> wrong?
>>>
>>> Thanks a lot,
>>> Lu
>>