http://confocal-microscopy-list.275.s1.nabble.com/Piezo-scanning-stage-induced-image-distortion-tp7584896p7584903.html
The ramp voltage looked fine on oscilloscope. I was using a photon counting
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> Have you looked at the ramp voltage on an oscilloscope to check for noise?
> What camera are you using (sCMOS, CCD)? are you decreasing the exposure
> when you increase the scanning frequency?
>
>
> Eric Marino
>
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>
>
>
> > On Mar 17, 2016, at 10:59 AM, Yan, Lu <
[hidden email]> wrote:
> >
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> > Hi Eric,
> >
> > The images are all taken at the fixed sample plane. The sample is a
> simple
> > grating pattern. I took these images at different scanning speed.
> >
> > Lu
> >
> > ------------------------------------------------------------------
> > -------------
> > Lu Yan
> > Nanostructured Fibers and Nonlinear Optics Laboratory
> > Electrical and Computer Engineering
> > Boston University
> > Rm. 505, 8 Saint Mary's St., Boston, MA 02215
> > +1.617.353.0286 (office) | 617.358.5917 (lab)
> >
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> >
> > On Thu, Mar 17, 2016 at 10:37 AM, Eric Marino <
[hidden email]>
> wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
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> posting.
> >> *****
> >>
> >> Hi Lu,
> >>
> >> Are the images a single plane or projected images?
> >>
> >> Eric Marino
> >>
[hidden email]
> >>
> >>
> >>
> >>> On Mar 17, 2016, at 10:08 AM, Yan, Lu <
[hidden email]> wrote:
> >>>
> >>> *****
> >>> To join, leave or search the confocal microscopy listserv, go to:
> >>>
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> >> posting.
> >>> *****
> >>>
> >>> Dear listers,
> >>>
> >>> I am encountering a problem with my piezo scanning stage (sample
> >> scanning)
> >>> in the microscopy setup. I found that at some discrete line scanning
> >>> frequencies (lines/s), the grating sample I imaged got some distortions
> >>> along the fast axis (the horizontal axis in following images). See this
> >>> link for some images:
> >>>
> >>
>
https://www.dropbox.com/s/9r0e2rea8oaepgi/Screenshot%20-%203_17_2016%20%2C%209_49_00%20AM.png?dl=0> >>>
> >>> As you can see, for example, at 30 Hz, it seemed that the grating was
> >>> splitting into multiple lines; and at 40Hz, they are also pretty bad. I
> >> am
> >>> using a bidirectional sawtooth voltage pattern to feed the piezo. So I
> >> was
> >>> wondering if anyone has similar experience on this? I switched to a new
> >>> scanning stage, but the distortion still existed, and it did not change
> >> as
> >>> field of view and pixel size. Does anyone have an ideal what might be
> >> wrong?
> >>>
> >>> Thanks a lot,
> >>> Lu
> >>
>