http://confocal-microscopy-list.275.s1.nabble.com/Piezo-scanning-stage-induced-image-distortion-tp7584896p7584904.html
> On Mar 17, 2016, at 3:37 PM, Yan, Lu <
[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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> *****
>
> Hi Eric,
>
> The ramp voltage looked fine on oscilloscope. I was using a photon counting
> module for the detection.
>
> Lu
>
> On 2016年3月17日 周四 at 下午1:18 Eric Marino <
[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>> Post images on
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>> *****
>>
>> Have you looked at the ramp voltage on an oscilloscope to check for noise?
>> What camera are you using (sCMOS, CCD)? are you decreasing the exposure
>> when you increase the scanning frequency?
>>
>>
>> Eric Marino
>>
[hidden email]
>>
>>
>>
>>> On Mar 17, 2016, at 10:59 AM, Yan, Lu <
[hidden email]> wrote:
>>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>>> Post images on
http://www.imgur.com and include the link in your
>> posting.
>>> *****
>>>
>>> Hi Eric,
>>>
>>> The images are all taken at the fixed sample plane. The sample is a
>> simple
>>> grating pattern. I took these images at different scanning speed.
>>>
>>> Lu
>>>
>>> ------------------------------------------------------------------
>>> -------------
>>> Lu Yan
>>> Nanostructured Fibers and Nonlinear Optics Laboratory
>>> Electrical and Computer Engineering
>>> Boston University
>>> Rm. 505, 8 Saint Mary's St., Boston, MA 02215
>>> +1.617.353.0286 (office) | 617.358.5917 (lab)
>>>
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>>> ------------------------------------------------------------------
>>> -------------
>>>
>>> On Thu, Mar 17, 2016 at 10:37 AM, Eric Marino <
[hidden email]>
>> wrote:
>>>
>>>> *****
>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>>>> Post images on
http://www.imgur.com and include the link in your
>> posting.
>>>> *****
>>>>
>>>> Hi Lu,
>>>>
>>>> Are the images a single plane or projected images?
>>>>
>>>> Eric Marino
>>>>
[hidden email]
>>>>
>>>>
>>>>
>>>>> On Mar 17, 2016, at 10:08 AM, Yan, Lu <
[hidden email]> wrote:
>>>>>
>>>>> *****
>>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>>>>> Post images on
http://www.imgur.com and include the link in your
>>>> posting.
>>>>> *****
>>>>>
>>>>> Dear listers,
>>>>>
>>>>> I am encountering a problem with my piezo scanning stage (sample
>>>> scanning)
>>>>> in the microscopy setup. I found that at some discrete line scanning
>>>>> frequencies (lines/s), the grating sample I imaged got some distortions
>>>>> along the fast axis (the horizontal axis in following images). See this
>>>>> link for some images:
>>>>>
>>>>
>>
https://www.dropbox.com/s/9r0e2rea8oaepgi/Screenshot%20-%203_17_2016%20%2C%209_49_00%20AM.png?dl=0>>>>>
>>>>> As you can see, for example, at 30 Hz, it seemed that the grating was
>>>>> splitting into multiple lines; and at 40Hz, they are also pretty bad. I
>>>> am
>>>>> using a bidirectional sawtooth voltage pattern to feed the piezo. So I
>>>> was
>>>>> wondering if anyone has similar experience on this? I switched to a new
>>>>> scanning stage, but the distortion still existed, and it did not change
>>>> as
>>>>> field of view and pixel size. Does anyone have an ideal what might be
>>>> wrong?
>>>>>
>>>>> Thanks a lot,
>>>>> Lu
>>>>
>>