Posted by
Joe Ping-Lin Hsiao on
URL: http://confocal-microscopy-list.275.s1.nabble.com/2-axes-mirror-calibration-tp7584945p7584950.html
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I am afraid that I was not clear in my first email. I am using a 2-axis
tilt mirror that has voltage controlled tilt. This mirror is set up to be
conjugate with the back focal plane of the microscope objective for point
illumination positioning within the specimen. When I specify an array of
points on a square grid in the voltage space of the 2 axis mirror, the
pattern of spots in the specimen plane shows that the axes are rotated and
are nonlinear. Unfortunately, I cannot physically rotate the mirror.
Therefore, I want to do all corrections for rotation and nonlinearity
through a software algorithm that maps the mirror voltage command space to
the 2d space of the specimen. Then I can use the reverse transform to
specify the needed voltages to achieve a desired beam path within the
specimen space. I would think that there are algorithms available perhaps
from the confocal laser scanning imaging literature, but have had trouble
locating these. Any help would be appreciated.
On Wed, Mar 30, 2016 at 5:07 PM, Joe Ping-Lin Hsiao <
[hidden email]>
wrote:
> Hi,
>
> I am using a 2-axis mirror to cast lasers onto specimens, and I am looking
> for a mathematical procedure to convert the voltage inputs of the mirror
> over the positions in the specimen space. Presumably it would take care of
> the rotation and nonlinearity.
>
> Thanks,
> Joe
>
>
>