Posted by
jerie on
URL: http://confocal-microscopy-list.275.s1.nabble.com/laser-alignment-issue-FluoView1000-tp7584981p7584991.html
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Listers,
according to suggestions from Olympus Americas and Avi Jacob (thanks!), I
have compared power measures with the internal light power meter (LPM) to
earlier measurements from August last year. All 6 lasers except the 405nm
line (39% but I noticed weaker signal and de-focus here before) show full
power (99-100%).
Both red LED's on the right side of the scan head are ON.
(filter turrets manual shutter open, filter block in mirror position1).
Still no light out of the scanhead.
All further suggestions welcome!
Very happy about the listserver!
Jens
Abraços,
Dr. Jens Rietdorf, visiting scientist @ center for technological
development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de Janeiro
Brasil.
On Mon, Apr 4, 2016 at 6:39 PM, jens rietdorf <
[hidden email]> wrote:
> Listers,
>
> following the installation of a new air condition, we are not succeeding
> to get any light after the scanhead of our Olympus FluoView1000 CLSM. End
> of fiber, there is plenty of light. Behind the scanhead, no light arrives.
> We work with an inverted IX 81 microscope.
>
> Unfortunately the service situation in Brazil is very poor, so if there
> were any suggestions how to proceed, I would rather try to check the
> alignment myself.
>
> Anyone having experience with the FluoView1000 scanhead?
>
> Thank you, Jens
>
> Dr. Jens Rietdorf, visiting scientist @ center for technological
> development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de Janeiro
> Brasil.
>