Posted by
Smith, Benjamin E. on
URL: http://confocal-microscopy-list.275.s1.nabble.com/laser-alignment-issue-FluoView1000-tp7584981p7584997.html
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One other thing that just came to mind that we see a bit on our FV500 (and could have been directly caused by the install) is that if the data cables going to the scan head or computer get pulled or jostled, then the scan head more or less stops working. This is often (but not always) indicated by the software failing to boot up the scan head altogether. That said, we have had one time where the scan head did boot, and the software allowed us to control the scan head. However, while we could get the arc lamp to shine out the objective, we could not get an image in laser scanning mode.
Almost every problem we've had with the FV500 has been fixed by replacing the problematic data cable. As such, I've now fastened the cables to fixed structures so that there is no tension in the system, and we have not seen this problem since (knock on wood).
-Ben Smith
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From: Confocal Microscopy List <
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Sent: Tuesday, April 5, 2016 12:08 PM
To:
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Subject: Re: laser alignment issue FluoView1000
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I'm not sure where the AOTF is in the FV1000 light path, but when an AOTF dies, the laser output drops nearly to zero (although an extremely faint beam is still transmitted). This may also be something to look into. However, the fact that both the UV and visible lines kicked the bucket, it seems unlikely to me that both AOTFs would die at the same time.
Another problem we've had on older systems is the filter turret/slider stops indexing properly, leading to little or no light depending on the filter position. Manually pushing the turret to a roughly centered position, and then letting the scan head reinitialize, correctly reset the indexing.
Along these lines, you should be able to see and hear each mechanical component in the scan head initialize. A failure of a mechanical component to correctly find its zero position (i.e. jitters side to side, moves to the extreme of the device's range, or doesn't move at all) is a quick way to determine if you may have a mechanical failure. What can sometimes happen is that a component rotated past it's expected range (effectively a negative position). As a result, if the system wasn't designed to understand a negative position, it will try and initialize the part by moving it further in the negative direction, leaving it in some extreme position (look up "integer overflow" for more info on this:
https://en.wikipedia.org/wiki/Integer_overflow).
This is why recentering the part can sometimes help, because the system can then correctly interpret the part's position and then zero it correctly when the scanhead is reinitialized. I found this problem tends to be especially predominant on older systems.
Good luck,
Ben Smith
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From: Confocal Microscopy List <
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Sent: Tuesday, April 5, 2016 9:38 AM
To:
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Subject: Re: laser alignment issue FluoView1000
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Hi,
in a normal point-scanning confocal there is no critical alignment in the
excitation path. I've seen come weird configurations (e.g. old Nikon PCM
2000), where both excitation light and the emission was directed through the
pinhole, there a small misalignment can result in complete loss of
excitation light. But I think FV1000 is not the case.
It must be either some safety shutter or the scanner is in parked position.
Can you hear the scanner?
Good luck!
zdenek
---------- Původní zpráva ----------
Od: jens rietdorf <
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Komu:
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Datum: 5. 4. 2016 10:25:29
Předmět: Re: laser alignment issue FluoView1000
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Listers,
according to suggestions from Olympus Americas and Avi Jacob (thanks!), I
have compared power measures with the internal light power meter (LPM) to
earlier measurements from August last year. All 6 lasers except the 405nm
line (39% but I noticed weaker signal and de-focus here before) show full
power (99-100%).
Both red LED's on the right side of the scan head are ON.
(filter turrets manual shutter open, filter block in mirror position1).
Still no light out of the scanhead.
All further suggestions welcome!
Very happy about the listserver!
Jens
Abraços,
Dr. Jens Rietdorf, visiting scientist @ center for technological
development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de Janeiro
Brasil.
On Mon, Apr 4, 2016 at 6:39 PM, jens rietdorf <
[hidden email]> wrote:
> Listers,
>
> following the installation of a new air condition, we are not succeeding
> to get any light after the scanhead of our Olympus FluoView1000 CLSM. End
> of fiber, there is plenty of light. Behind the scanhead, no light arrives.
> We work with an inverted IX 81 microscope.
>
> Unfortunately the service situation in Brazil is very poor, so if there
> were any suggestions how to proceed, I would rather try to check the
> alignment myself.
>
> Anyone having experience with the FluoView1000 scanhead?
>
> Thank you, Jens
>
> Dr. Jens Rietdorf, visiting scientist @ center for technological
> development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de
Janeiro
> Brasil.
>"