Posted by
Jacqueline Ross on
URL: http://confocal-microscopy-list.275.s1.nabble.com/laser-alignment-issue-FluoView1000-tp7584981p7585007.html
Hi Jens,
To me, it doesn't sound like alignment per se as you don't have any light from any lasers.
Did you have to dismantle the confocal in order to have the A/C installed. If so, there might be a polariser somewhere in the scan head that's affecting things. We had a laser replaced and the engineer couldn't get any light out of the lens initially though everything was fine on the fibre side. Then it turned out that there was a polariser in the system (don't know where sorry:-() that was blocking all of the light!
The other thing I thought I'd mention (though am a bit embarrassed to do so) is that we often have people turning off the keys on our scanner power supplies. There's always a bit of puzzlement that the system won't scan since the indicator light does come on with the switch even when the keys are in the OFF position.
Checking the cables between the scanner power supplies and scan head as has been suggested is a very good idea too though we've never had any problems with that as ours are all at the back.
Kind regards,
Jacqui
Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND
Tel: 64 9 923 7438
Fax: 64 9 373 7484
http://www.fmhs.auckland.ac.nz/sms/biru/-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of jens rietdorf
Sent: Tuesday, 5 April 2016 9:40 a.m.
To:
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Subject: laser alignment issue FluoView1000
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Listers,
following the installation of a new air condition, we are not succeeding to
get any light after the scanhead of our Olympus FluoView1000 CLSM. End of
fiber, there is plenty of light. Behind the scanhead, no light arrives. We
work with an inverted IX 81 microscope.
Unfortunately the service situation in Brazil is very poor, so if there
were any suggestions how to proceed, I would rather try to check the
alignment myself.
Anyone having experience with the FluoView1000 scanhead?
Thank you, Jens
Dr. Jens Rietdorf, visiting scientist @ center for technological
development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de Janeiro
Brasil.