Re: single molecule bleaching comparison

Posted by lechristophe on
URL: http://confocal-microscopy-list.275.s1.nabble.com/single-molecule-bleaching-comparison-tp7585057p7585058.html

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Hi,

Two references I'm aware of that compare properties of fluorophores for
single molecule imaging (including bleaching characteristics):

http
<http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0074200>
://
<http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0074200>
journals.plos.org
<http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0074200>/
<http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0074200>
plosone
<http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0074200>
/article?
<http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0074200>id
<http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0074200>
=10.1371/
<http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0074200>
journal.pone.0074200
<http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0074200>

http <http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142238/>://
<http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142238/>www.ncbi.nlm.nih.gov
<http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142238/>/
<http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142238/>pmc
<http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142238/>/articles/PMC4142238/
<http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142238/>

But I don't think they compare the green Alexa and Atto dyes.

Also related, but for FPs:
http <http://www.ncbi.nlm.nih.gov/pubmed/25762325>://
<http://www.ncbi.nlm.nih.gov/pubmed/25762325>www.ncbi.nlm.nih.gov
<http://www.ncbi.nlm.nih.gov/pubmed/25762325>/
<http://www.ncbi.nlm.nih.gov/pubmed/25762325>pubmed
<http://www.ncbi.nlm.nih.gov/pubmed/25762325>/25762325
<http://www.ncbi.nlm.nih.gov/pubmed/25762325>

Christophe
Le 15 avr. 2016 6:47 PM, "Jeff Spector" <[hidden email]> a écrit :

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>
> Greetings,
>   Can anyone point me to a nice reference comparing the bleaching
> times/brightness of the Alexa dyes vs the Atto dyes (in particular Alexa
> 488 vs Atto 488). I've found many references that use them for storm and
> compare their blinking properties but I'm interested in their bleaching
> times. They appear to have about the same brightest, so I'm interested in
> whether one of them will last longer in a single molecule experiment. I
> realize this is very set up dependent laser power objective and so on but
> has anyone compared their bleaching under the same conditions?  Can someone
> point me towards a reference (or even anecdotal knowledge)...
> Thanks
>