Re: Ratiometric measurements for multiphoton microscopy

Posted by mmodel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Ratiometric-measurements-for-multiphoton-microscopy-tp7585091p7585092.html

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Konstantin,
I would look probes that have common excitation and a shift in emission (Indo-1 for example)

Mike Model

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From: Confocal Microscopy List <[hidden email]> on behalf of Microscopia-IBIS <[hidden email]>
Sent: Monday, April 25, 2016 5:32 AM
To: [hidden email]
Subject: Ratiometric measurements for multiphoton microscopy

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Dear all.



One of our groups is interested in doing fluorescence ratiometric
measurements (e.g., FURA-2, ROS) using a multiphoton microscopy system. From
what we understand the laser tuning speeds won’t be fast enough to switch
between two different excitation wavelengths, and we were wondering if there
could be ways to maintain these ratiometric attributes while still
performing a laser two-photon excitation. Any ideas or suggestions will be
very much appreciated.



Thanks and regard,

Dr. Konstantín Levitskiy

Servicio de Microscopía

InstitutodeBiomedicinadeSevilla - IBiS

Campus del Hospital Universitario Virgen del Rocío

Avda. Manuel Siurot s/nº

41013 Sevilla

Tlfno: 955 92 3030

Email: [hidden email]

Web: www.ibis-sevilla.es





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