widefield getting better images than spinning disk

Posted by RJ3 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/widefield-getting-better-images-than-spinning-disk-tp7585177.html

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This has me stumped. I suspected it was a lack of light but I turned up the
excitation light significantly and still haven't gotten good images with the
spinning disk in place. The widefield mode is giving much better images
which doesn't make any sense to me. I figured I'd ask for recommendations in
case anyone has been in a similar boat, especially with the hardware I'm
running:

Nikon Ti w/ Crest X-Light v1 spinning disk. Detector is a Andor Zyla 4.2
PLUS sCMOS.