Re: widefield getting better images than spinning disk

Posted by samjlord on
URL: http://confocal-microscopy-list.275.s1.nabble.com/widefield-getting-better-images-than-spinning-disk-tp7585177p7585188.html

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On Fri, 20 May 2016 12:46:37 -0400, Rafael Jaimes III <[hidden email]>
wrote:
>1. Live neonatal rat cardiomyocyte-fibroblast layers stained with Fluo-4
>for calcium. They are field stimulated and imaged >100 fps.

At 10 ms exposure time, I doubt you're getting enough fluorescence hitting the
camera to get a sufficient signal to noise. Wide field is always much brighter than
confocal because both the excitation light and the emission intensity is reduced
going through the pinholes.

Why don't you send images of wide field vs confocal so we can see what you mean
by "better."