Re: widefield getting better images than spinning disk

Posted by RJ3 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/widefield-getting-better-images-than-spinning-disk-tp7585177p7585190.html

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Hi Andrea,

Thanks for your reply! It means a lot to hear from the president of the
company.

I was also wondering -- is there an easy way to determine the maximum
acquisition speed for my disk? I have the 10,000 RPM variant. One of the
other users mentioned that 10ms exposure / 100 fps may be too fast, at
least regarding the light levels.​ Do you typically use EMCCD for these
disks to accommodate the loss of light? I am worried that my sCMOS is not
sensitive enough.

As some others requested, I will certainly post some pictures to imgur when
I get a chance.. please stand by.

Rafael

On Fri, May 20, 2016 at 2:45 PM, Andrea Latini <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Rafael,
> thank you for raising the question to the Confocal List and sorry for my
> late response. Luckily
> John Oreopoulos answers/explanations are a very good starting point on how
> to optimize your
> confocal system and better evaluate your experimental results and of
> course I fully agree with
> him.
> I'll be pleased to help you on pinholes focusing and overall system setup
> and to directly
> evaluate your acquisitions.
>
> regards.
>
> Andrea Latini
> President
> CrestOptics srl
>