Re: widefield getting better images than spinning disk

Posted by samjlord on
URL: http://confocal-microscopy-list.275.s1.nabble.com/widefield-getting-better-images-than-spinning-disk-tp7585177p7585197.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

I would recommmend setting the exposure time to 100 ms or however long an exposure it
takes to get a good image. If the image gets bright but the contrast still looks worse than
wide field, then maybe there's an alignment issue. But I suspect your sample is just too dim
to image with confocal at 10 ms per frame. Not many samples are bright enough for that.
Remember that confocal is designed to throw out light in order to improve optical slicing.