Re: [QUAR] Re: widefield getting better images than spinning disk

Posted by Nico Stuurman-3 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/widefield-getting-better-images-than-spinning-disk-tp7585177p7585206.html

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Dear Andrea,

> collection efficiency CF/WF is 5% to 6% in our systems with 70um pinholes (fill factor 8.2%).

Could you please explain what that number means, or better, how it is
measured?  The way it is written, I have the impression that when a very
thin film of fluorescent material is excited by the same amount of light
you get 5-6% intensity in confocal mode and 100% in wide-field mode.  
Obviously, that would be pretty bad (and according to the discussion on
this list, you should not lose more than a few percent of the
in-focus-signal through the pinholes), so I assume that you mean
something else.

Best,

Nico