Re: 3d printers for microsocpy

Posted by Philip Nicovich on
URL: http://confocal-microscopy-list.275.s1.nabble.com/3d-printers-for-microsocpy-tp7585214p7585233.html

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Craig,

Right now the silicone rubber gasket seals between the bottom of the
chamber 'top' and the coverslip.  The well holding media and the sample,
from the bottom-up, is then coverslip glass - silicone gasket - 3D printed
plastic.  I am not picturing how I can get away from using some sort of
soft gasket between the plastic and coverslip and getting a good seal.  The
3D printed parts simply aren't smooth enough.  With the conical vial the
plastic of the vial can distort somewhat if you tighten it all down hard
and provide a seal but if you tried that with a coverslip it'd just snap.
Teflon tape would take up the slack in the threaded part well, but again
it's that glass-plastic transition that's harder.  Maybe there's another
geometry for these that would move away from that being the critical joint?

Thanks,
Rusty



On Wed, May 25, 2016 at 2:48 PM, Craig Brideau <[hidden email]>
wrote:

> *****
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> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Maybe make the tolerances on the threads slightly looser and use Teflon
> tape to reinforce the seal?
>
> Craig
> On May 24, 2016 6:06 PM, "Rusty Nicovich" <[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Sergey,
> >
> > We haven't tried threaded chamber junctions.  It's an intriguing idea,
> > though.  I'm a little worried that the printing tolerances and surface
> > finish wouldn't be sufficient to provide a good seal, but at the same
> time
> > I did 3D print a cap for 15 mL conical vials for another project that was
> > water-tight.
> >
> > Will certainly give it a shot when I get some free time.
> >
> > Thanks,
> > Rusty
> >
> > On Wed, May 25, 2016 at 9:56 AM, Sergey Tauger <[hidden email]>
> wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > Post images on http://www.imgur.com and include the link in your
> > posting.
> > > *****
> > >
> > > Hello Rusty,
> > >
> > > Did you try taper (conical) tread between top and bottom parts? It will
> > > allow get rid of
> > > magnets and probably silicon rubber. I saw teflon chambers with taper
> > > thread, made using
> > > CNC app 20 years ago, they still perform good enough.
> > >
> > > Best,
> > > Sergey
> > >
> >
>

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*Philip R Nicovich*

*Research Fellow,  **ARC Centre of Excellence in Advanced Molecular Imaging*



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