Posted by
jerie on
URL: http://confocal-microscopy-list.275.s1.nabble.com/laser-alignment-issue-FluoView1000-tp7584981p7585263.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
Dear colleagues,
thank you all for your help on the issue, it turned out a shutter inside
the scanhead was blocked. This problem was relatively rapidly addressed by
the service of Olympus Brasil and Olympus Americas. Very helpful input for
the differential diagnosis came from Farid Jalali of Olympus Canada.
A new (maybe related) issue occurred now, we experience very low
sensitivity on detector channels 1&2,
channel 3 operates normally. the effect appears to be stronger in the
blue/green part of the spectrum than for the red.
The FV diagnostic tool does not indicate any abnormalities.
Again, all ideas and suggestions are very welcome.
Kind regards, Jens
Dr. Jens Rietdorf, visiting scientist @ center for technological
development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de Janeiro
Brasil.
On Mon, Apr 4, 2016 at 6:39 PM, jens rietdorf <
[hidden email]> wrote:
> Listers,
>
> following the installation of a new air condition, we are not succeeding
> to get any light after the scanhead of our Olympus FluoView1000 CLSM. End
> of fiber, there is plenty of light. Behind the scanhead, no light arrives.
> We work with an inverted IX 81 microscope.
>
> Unfortunately the service situation in Brazil is very poor, so if there
> were any suggestions how to proceed, I would rather try to check the
> alignment myself.
>
> Anyone having experience with the FluoView1000 scanhead?
>
> Thank you, Jens
>
> Dr. Jens Rietdorf, visiting scientist @ center for technological
> development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de Janeiro
> Brasil.
>