Re: laser alignment issue FluoView1000

Posted by jerie on
URL: http://confocal-microscopy-list.275.s1.nabble.com/laser-alignment-issue-FluoView1000-tp7584981p7585266.html

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Hi Glen,

exactly that was what I did to find out detector 1&2 are far weaker than
ch3. A good guess seems to be 'Spectral Detectors out of tune' which was
the remote diagnosis by Olympus that just arrived.

Anyone with experience in tuning Spectral Detectors on the FV1000?

Thanks a lot,
jens

Abraços,

Dr. Jens Rietdorf, visiting scientist @ center for technological
development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de Janeiro
Brasil.

On Wed, Jun 1, 2016 at 3:34 PM, Glen MacDonald <[hidden email]>
wrote:

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> Hi Jens,
> Have you tried redirecting the blue and green channels to the detector
> that is usually “red”?
> after ~28,000 hrs on our FV-1000, the “green” channel started dropping
> out, sometimes entirely, other times with dark bands in the scanned frame.
> The problem persisted when routed to other detectors.  The culprit was the
> AOTF.
>
> Good luck,
> Glen
> Glen MacDonald
> Digital Microscopy Center
> Box 357923
> University of Washington
> Seattle, WA 98195-7923  USA
> [phone]
> [hidden email]
>
>
>
>
>
>
>
>
> > On Jun 1, 2016, at 10:35 AM, jens rietdorf <[hidden email]> wrote:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear colleagues,
> >
> > thank you all for your help on the issue, it turned out a shutter inside
> > the scanhead was blocked. This problem was relatively rapidly addressed
> by
> > the service of Olympus Brasil and Olympus Americas. Very helpful input
> for
> > the differential diagnosis came from Farid Jalali of Olympus Canada.
> >
> > A new (maybe related) issue occurred now, we experience very low
> > sensitivity on detector channels 1&2,
> > channel 3 operates normally. the effect appears to be stronger in the
> > blue/green part of the spectrum than for the red.
> > The FV diagnostic tool does not indicate any abnormalities.
> >
> > Again, all ideas and suggestions are very welcome.
> >
> > Kind regards, Jens
> >
> > Dr. Jens Rietdorf, visiting scientist @ center for technological
> > development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de
> Janeiro
> > Brasil.
> >
> > On Mon, Apr 4, 2016 at 6:39 PM, jens rietdorf <[hidden email]>
> wrote:
> >
> >> Listers,
> >>
> >> following the installation of a new air condition, we are not succeeding
> >> to get any light after the scanhead of our Olympus FluoView1000 CLSM.
> End
> >> of fiber, there is plenty of light. Behind the scanhead, no light
> arrives.
> >> We work with an inverted IX 81 microscope.
> >>
> >> Unfortunately the service situation in Brazil is very poor, so if there
> >> were any suggestions how to proceed, I would rather try to check the
> >> alignment myself.
> >>
> >> Anyone having experience with the FluoView1000 scanhead?
> >>
> >> Thank you, Jens
> >>
> >> Dr. Jens Rietdorf, visiting scientist @ center for technological
> >> development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de
> Janeiro
> >> Brasil.
> >>
>