Advice sought for imaging Metasystems 24XCyte DNA Probe Kit

Posted by Andrew Vaughan-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Advice-sought-for-imaging-Metasystems-24XCyte-DNA-Probe-Kit-tp7585292.html

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Hello All,

Does anyone have any experience imaging Metasystems 24XCyte DNA Probe Kit (or any other Metasystems kit) using microscopes other than the company's own Isis imaging system? The kit allows identification of the 24 human chromosomes by painting them with combinations of 5 different fluorophores. There is significant spectral overlap so the recommended filter sets have narrow bands. I would like to know whether anyone has tried imaging these (or other similar chromosome painting products and protocols) using other filter sets or confocal microscopes and perhaps using spectral imaging and un-mixing to separate the fluorophores.

Here's the link to Metasystems page:

http://www.metasystems-international.com/index.php?option=com_joodb&view=article&joobase=5&id=1%3Ad-0125-060-di&Itemid=242

Any advice would be much appreciated.

Thanks,

Andrew Vaughan.