Re: camera for fluorescence <$4000

Posted by Michael Giacomelli on
URL: http://confocal-microscopy-list.275.s1.nabble.com/camera-for-fluorescence-4000-tp7585340p7585343.html

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Point Grey periodically updates their evaluation of cost-effective sensors:

https://www.ptgrey.com/camera-sensor-review

The newly added IMX264/IMX265 series sensors obtain a read noise of about
2.3 electrons and an absolute sensitivity of about 4 photons.  sCMOS will
do better (read noise of about 1 electron on the newest models).  From the
brightness of your specimen you can get a rough idea how well that will
work for you.

Mike


On Thu, Jun 16, 2016 at 4:19 PM, Craig Brideau <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I've used a 'machine vision' grade camera for basic fluorescence microscopy
> with decently bright samples with some success. They are meant for robotic
> sorting applications and the like so they give a decent image, although
> they are lacking in sensitivity compared to a 'real' microscopy camera. If
> you can see it clearly by eye though this type of camera should be
> adequate.
>
> Craig
>
> On Thu, Jun 16, 2016 at 1:26 PM, Aryeh Weiss <[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > I was asked if I can recommend a camera for fluorescence microscopy for
> > under $4k.
> > It is not single molecule, but if they can see it, they would like the
> > camera to see it.
> >
> > I found a similar question on the list from 2014
> >
> >
> https://lists.umn.edu/cgi-bin/wa?A2=ind1403&L=confocalmicroscopy&P=R18770&1=confocalmicroscopy&9=A&J=on&d=No+Match%3BMatch%3BMatches&z=4
> >
> > but this is a moving target, and my price constraint is higher, so I am
> > wondering what else is out there.
> >
> > Are there any sCMOS  cameras that qualify? Does anyone have experience
> > with the less known brands (eg cameras geared toward amateur astronomy)?
> > I have a strong preference for cameras that will work with micro-manager.
> > I would appreciate finding out what solutions people on the list have
> > found.
> >
> > Thanks in advance
> > --aryeh
> >
> > --
> > Aryeh Weiss
> > Faculty of Engineering
> > Bar Ilan University
> > Ramat Gan 52900 Israel
> >
> > Ph:  972-3-5317638
> > FAX: [phone]
> >
>