Re: camera for fluorescence <$4000

Posted by Aryeh Weiss on
URL: http://confocal-microscopy-list.275.s1.nabble.com/camera-for-fluorescence-4000-tp7585340p7585346.html

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Many thanks to the many people who already responded to my inquiry.

On 16/06/2016 11:51 PM, Armstrong, Brian wrote:
> Hi Aryeh, I think that you can get a good scientific camera for that much from Q Imaging. Thor Labs also has CCD cameras around this price point.
> I inherited 4 astronomy cameras and was very disappointed with them and ended up just giving them away
I also heard off-list from Q-Imaging, who suggested their R1 (1.4MP CCD,
cooled). I suspect that in Israel it will too expensive, but we are
checking that.
Thanks for the comment on the astronomy cameras.

On 17/06/2016 12:35 AM, Pedro Almada wrote:
>
> Hi Aryeh, have you had a look at the Hamamatsu flash 2.8?
>
This is an attractive option, but I am pretty sure it will be too
expensive (we will check). I know that the Flash 4.o costs around $9k in
Israel. What does it cost in your area?

On 17/06/2016 12:56 AM, Michael Giacomelli wrote:
> Point Grey periodically updates their evaluation of cost-effective sensors:
>
> https://www.ptgrey.com/camera-sensor-review
>
> The newly added IMX264/IMX265 series sensors obtain a read noise of about
> 2.3 electrons and an absolute sensitivity of about 4 photons.  sCMOS will
> do better (read noise of about 1 electron on the newest models).  From the
> brightness of your specimen you can get a rough idea how well that will
> work for you.

I have a Pt Grey Chamelian, but I found that I cannot easily use it with
Micromanager. It claims to run with IIDC (DC1394), and it does, sort of.
However, at least in my hands, it was difficult, and not all of the
modes worked in micromanager (although it worked ok with coriander ,
which is a program used for testing such things).

On 17/06/2016 2:01 AM, Ochoa, Lorenzo F. wrote:
> Hello Aryeh,
>
> I've had good results from the Thorlabs High Sensitivity USB 3.0 CMOS Cameras. They are relatively cheap ($1.5K) and are compatible with Micro-Manager: https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=4024
This looks interesting. The price is right, at the cost of being
uncooled (but cooling for exposures under a second may not be critical).
It is supposed to work with micromanager.

Is anybody using DSLR cameras for fluorescence imaging?  I know they are
color and probably less sensitive, but I still wonder...

Again thanks to the many people who replied.

--aryeh





On 16/06/2016 10:26 PM, Aryeh Weiss wrote:

> I was asked if I can recommend a camera for fluorescence microscopy
> for under $4k.
> It is not single molecule, but if they can see it, they would like the
> camera to see it.
>
> I found a similar question on the list from 2014
> https://lists.umn.edu/cgi-bin/wa?A2=ind1403&L=confocalmicroscopy&P=R18770&1=confocalmicroscopy&9=A&J=on&d=No+Match%3BMatch%3BMatches&z=4 
>
>
> but this is a moving target, and my price constraint is higher, so I
> am wondering what else is out there.
>
> Are there any sCMOS  cameras that qualify? Does anyone have experience
> with the less known brands (eg cameras geared toward amateur astronomy)?
> I have a strong preference for cameras that will work with micro-manager.
> I would appreciate finding out what solutions people on the list have
> found.
>
> Thanks in advance
> --aryeh
>

--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]