http://confocal-microscopy-list.275.s1.nabble.com/camera-for-fluorescence-4000-tp7585340p7585356.html
read noise. I suspect the problem with dSLRs is that most are designed
> Thank you for your reply.
>
> On 17/06/2016 7:10 PM, Michael Giacomelli wrote:
>
>> I also heard off-list from Q-Imaging, who suggested their R1 (1.4MP CCD,
>>>
>> cooled).
>>
>> For what it's worth, the read noise on that camera is worse than many of
>> the inexpensive machine vision cameras Edmunds or Thorlabs sell. Unless
>> you must have cooling, it may not be a great choice.
>>
> The R1 spec sheet (
> www.qimaging.com/products/datasheets/Retiga-R1-Datasheet.pdf ) claims 7
> electrons for the read noise.
> I have not been able to find the read noise specification for the machine
> vision cameras.
> Do you have that information, or is there a different parameter from which
> RMS read noise can be derived?
>
> Is anybody using DSLR cameras for fluorescence imaging? I know they are
>>>
>> color and probably less sensitive, but I still wonder...
>>
>> I've seen these used a lot in medical labs for taking pictures of slides.
>> The main problem with a dSLR (besides the software interface) is that the
>> sensor form factor is usually very large. For example, that R1 you linked
>> above uses a Sony CCD sensor with an 11 mm diagonal, whereas a typical
>> dSLR
>> sensor format is 25-30 mm diagonal. To maintain the same field of view
>> you
>> would either have to change the tube lens or get a ~3:1 expansion
>> telescope. For comparable performance it usually makes more sense to just
>> buy a camera with the right sensor size.
>>
>> I was thinking of dslr cameras with aps-c sensors (which cost much less
> than full frame dslr),
> but even then you are correct that a zoom will be needed to fill the
> sensor.
> However, such adapters are available for under $1000, and the cameras
> themselves are under $500.
> Nikon makes 2.5x F-mount adapter for their DS-Qi2 (which uses a full
> frame sensor) which costs about 450 Euro.
> So if the dslr has sufficient sensitivity, it can be worth it.
>
> Best regards
> --aryeh
>
>
>> On Fri, Jun 17, 2016 at 1:07 AM, Aryeh Weiss <
[hidden email]> wrote:
>>
>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>>> Post images on
http://www.imgur.com and include the link in your
>>> posting.
>>> *****
>>>
>>> Many thanks to the many people who already responded to my inquiry.
>>>
>>> On 16/06/2016 11:51 PM, Armstrong, Brian wrote:
>>>
>>> Hi Aryeh, I think that you can get a good scientific camera for that much
>>>> from Q Imaging. Thor Labs also has CCD cameras around this price point.
>>>> I inherited 4 astronomy cameras and was very disappointed with them and
>>>> ended up just giving them away
>>>>
>>>> I also heard off-list from Q-Imaging, who suggested their R1 (1.4MP CCD,
>>> cooled). I suspect that in Israel it will too expensive, but we are
>>> checking that.
>>> Thanks for the comment on the astronomy cameras.
>>>
>>> On 17/06/2016 12:35 AM, Pedro Almada wrote:
>>>
>>> Hi Aryeh, have you had a look at the Hamamatsu flash 2.8?
>>>>
>>>> This is an attractive option, but I am pretty sure it will be too
>>>>
>>> expensive (we will check). I know that the Flash 4.o costs around $9k in
>>> Israel. What does it cost in your area?
>>>
>>> On 17/06/2016 12:56 AM, Michael Giacomelli wrote:
>>>
>>> Point Grey periodically updates their evaluation of cost-effective
>>>> sensors:
>>>>
>>>>
https://www.ptgrey.com/camera-sensor-review>>>>
>>>> The newly added IMX264/IMX265 series sensors obtain a read noise of
>>>> about
>>>> 2.3 electrons and an absolute sensitivity of about 4 photons. sCMOS
>>>> will
>>>> do better (read noise of about 1 electron on the newest models). From
>>>> the
>>>> brightness of your specimen you can get a rough idea how well that will
>>>> work for you.
>>>>
>>>> I have a Pt Grey Chamelian, but I found that I cannot easily use it with
>>> Micromanager. It claims to run with IIDC (DC1394), and it does, sort of.
>>> However, at least in my hands, it was difficult, and not all of the modes
>>> worked in micromanager (although it worked ok with coriander , which is a
>>> program used for testing such things).
>>>
>>> On 17/06/2016 2:01 AM, Ochoa, Lorenzo F. wrote:
>>>
>>> Hello Aryeh,
>>>>
>>>> I've had good results from the Thorlabs High Sensitivity USB 3.0 CMOS
>>>> Cameras. They are relatively cheap ($1.5K) and are compatible with
>>>> Micro-Manager:
>>>>
https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=4024>>>>
>>>> This looks interesting. The price is right, at the cost of being
>>> uncooled
>>> (but cooling for exposures under a second may not be critical). It is
>>> supposed to work with micromanager.
>>>
>>> Is anybody using DSLR cameras for fluorescence imaging? I know they are
>>> color and probably less sensitive, but I still wonder...
>>>
>>> Again thanks to the many people who replied.
>>>
>>> --aryeh
>>>
>>>
>>>
>>>
>>>
>>>
>>> On 16/06/2016 10:26 PM, Aryeh Weiss wrote:
>>>
>>> I was asked if I can recommend a camera for fluorescence microscopy for
>>>> under $4k.
>>>> It is not single molecule, but if they can see it, they would like the
>>>> camera to see it.
>>>>
>>>> I found a similar question on the list from 2014
>>>>
>>>>
>>>>
https://lists.umn.edu/cgi-bin/wa?A2=ind1403&L=confocalmicroscopy&P=R18770&1=confocalmicroscopy&9=A&J=on&d=No+Match%3BMatch%3BMatches&z=4>>>>
>>>> but this is a moving target, and my price constraint is higher, so I am
>>>> wondering what else is out there.
>>>>
>>>> Are there any sCMOS cameras that qualify? Does anyone have experience
>>>> with the less known brands (eg cameras geared toward amateur astronomy)?
>>>> I have a strong preference for cameras that will work with
>>>> micro-manager.
>>>> I would appreciate finding out what solutions people on the list have
>>>> found.
>>>>
>>>> Thanks in advance
>>>> --aryeh
>>>>
>>>>
>>>> --
>>> Aryeh Weiss
>>> Faculty of Engineering
>>> Bar Ilan University
>>> Ramat Gan 52900 Israel
>>>
>>> Ph: 972-3-5317638
>>> FAX: 972-3-7384051
>>>
>>>
>
> --
> Aryeh Weiss
> Faculty of Engineering
> Bar Ilan University
> Ramat Gan 52900 Israel
>
> Ph: 972-3-5317638
> FAX: 972-3-7384051
>
>