Posted by
Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Chromosome-painting-tp7585339p7585361.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
Camilla,
there is a rather large body of literature on FISH in interphase nuclei.
If you search Pubmed for contributions by the groups of Wendy Bickmore,
Thomas Cremer, or Tom Misteli, this would be a good starting point.
There are also several papers or protocols on 'how-to' or 'how not to', eg.
https://www.ncbi.nlm.nih.gov/pubmed/18951171https://www.ncbi.nlm.nih.gov/pubmed/16954680and maybe one of my own:
https://www.ncbi.nlm.nih.gov/pubmed/17119992'Back in the days', I always used non-commercial, nick-translation
labeled probes. I would assume this may have changed in the mean time, I
am currently not working in this field. Whether you are using a BAC
clone or a chromosome paint as probe does not make a principal
difference. you just need more DNA for the latter.
Steffen
Am 16.06.2016 um 15:02 schrieb Camilla Margaroli:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Hello everyone,
>
>
> I was wondering if any of you has ever done chromosome painting to see the chromosome location in the nucleus. If yes, could you let me know which probes did you use and if you did it yourself or if you sent it elsewhere to do it?
>
> It will be my first time doing this staining.
>
> Thanks for your help!
>
> Best,
>
> Camilla
>
--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging
Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany
http://www.bioimaging.bmc.med.uni-muenchen.de