Posted by
mcammer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/laser-choices-for-multiphoton-tp7585404.html
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We are considering a multiphoton purchase and a big issue is illumination. Putting budget constraints aside and thinking purely of imaging non-destructively in live tissues, what would give us the most flexibility for exciting multiple probes for maximum signal while minimally damaging the biology?
We are used to using lasers that tune from approx 700 to 1060 nm and mostly use 890 to 930 nm, but this does not provide good red imaging. If money were no issue, would we be wise to get a laser such as the dual line Insight or two lasers from 690 to 1080 nm?
Some of the questions that have come up are:
* We think a laser that tunesup to 1300 nm would solve the red imaging problem, but for bluer probes (CFP, GFP, etc) & second harmonics of collagen would we need to use a different wavelength? If so, how long does this take and do the commercial systems support this?
* With a dual line laser that tunes out to 1300 nm is the fixed 1046 nm line really useful?
* Does a dual line system cook the sample?
Any thoughts on this (and on specific multiphoton scopes) greatly appreciated.
Thank you!
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Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center
Cell: 914-309-3270 Office: Skirball 2nd Floor main office
http://ocs.med.nyu.edu/microscopy &
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