Posted by
George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/laser-choices-for-multiphoton-tp7585404p7585405.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
Hi Michael,
How about Coherent Chameleon OPO-Vis or MPX? (or equivalent from other
vendors).
https://www.coherent.com/products/?1642/Chameleon-Compact-OPO-Family*New: Chameleon MPX* extends the wavelength range of Chameleon Vision
and Ultra Ti:Sapphire lasers and is specifically designed and optimized
for non-linear imaging techniques.
Employing is the latest generation fan-poled OPO technology; the fully
automated Chameleon MPX delivers high peak power to the sample plane
with short pulse pulses and dispersion compensation optimized for
typical commercial microscope systems.
Featuring a wide pump tuning range, the Chameleon MPX offers
independently tunable dual beam excitation of popular fluorescent
markers (eg. eGFP, mCherry), enabling powerful and truly flexible
multimodal imaging
*Chameleon Compact OPO* Long wavelength tuning capability up to 1600
nm, with idler option to extend to 4000 nm. Designed for simplicity and
ease-of-use by non-laser experts, Compact OPO is a truly “black box”
wavelength extension. No adjustment is required during tuning and the
OPO can be controlled via USB or RS232 link.
*Chameleon Compact OPO-Vis* provides automated frequency doubling of the
OPO and laser output.. Used in conjunction with the Compact OPO and
Chameleon pump lasers, it gives access to an unprecedented automated
tuning range of 340 nm to 1600 nm, with simple touch screen controls and
no manual intervention. This OPO provides hundreds of milliwatts of
output power over most of its tuning range that can be expanded to cover
also the mid IR region between 1750 nm and 4000 nm.
The visible wavelengths could be used at low power for 1p excitation
down to 340 nm (no need for vis lasers), less phototoxicity, either for
(examples):
* Fura-2 (+Ca++)
* Brilliant Ultraviolet ... and ~395 nm for Brilliant Violet's (and
longer wavelengths for Brilliant Blue's)
* NIRvana Sciences chlorins ...
http://nirvanasciences.com/?page_id=3088* NADH, NADPH (see below).
MPEF may be better for NADH, and with a new fluorescent protein
biosensor for NAD+ along with existing NADH FP biosensors, direct
excitation of NADH may not matter any more (since the FP's may be
brighter and can localize to a compartment of interest).
There is potential to shape the beam(s) to get 2 or more STED doughnuts
at different wavelengths (and I think the plate can go where the DIC
Wollaston prism would go). See
https://works.bepress.com/gmcnamara/3/https://works.bepress.com/gmcnamara/13/... there is a newer Q-plate for STED
https://www.osapublishing.org/abstract.cfm?uri=cleo_si-2015-STu1L.5//
Chris Xu (Cornell Univ) has published on using very long wavelengths
http://www.ncbi.nlm.nih.gov/pubmed/25909022 ... 1700 nm
http://www.ncbi.nlm.nih.gov/pubmed/25360361 ... 2p, 3p, 4p using 800,
1300 and 1680 nm
best wishes,
George
On 7/7/2016 11:54 AM, Cammer, Michael wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> We are considering a multiphoton purchase and a big issue is illumination. Putting budget constraints aside and thinking purely of imaging non-destructively in live tissues, what would give us the most flexibility for exciting multiple probes for maximum signal while minimally damaging the biology?
>
>
>
> We are used to using lasers that tune from approx 700 to 1060 nm and mostly use 890 to 930 nm, but this does not provide good red imaging. If money were no issue, would we be wise to get a laser such as the dual line Insight or two lasers from 690 to 1080 nm?
>
>
>
> Some of the questions that have come up are:
>
> * We think a laser that tunesup to 1300 nm would solve the red imaging problem, but for bluer probes (CFP, GFP, etc) & second harmonics of collagen would we need to use a different wavelength? If so, how long does this take and do the commercial systems support this?
>
> * With a dual line laser that tunes out to 1300 nm is the fixed 1046 nm line really useful?
>
> * Does a dual line system cook the sample?
>
>
>
> Any thoughts on this (and on specific multiphoton scopes) greatly appreciated.
>
>
>
> Thank you!
>
>
> =========================================================================
> Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center
> Cell: 914-309-3270 Office: Skirball 2nd Floor main office
>
http://ocs.med.nyu.edu/microscopy &
http://microscopynotes.com/>
>
> ------------------------------------------------------------
> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
> =================================
--
George McNamara, PhD
Houston, TX 77054
[hidden email]
https://www.linkedin.com/in/georgemcnamarahttps://works.bepress.com/gmcnamara/75/