Posted by
Martin Wessendorf-2 on
Jul 08, 2016; 8:10pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Stray-light-in-TIRF-microscopy-tp7585414.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
[Posted on behalf of Dr. Alexander Asanov.]
Dear colleagues,
Are you aware of quantitative data for the intensity of stray light in
TIRF microscopy? I found only one paper by A. Mattheyses and D. Axelrod,
2006, which quantitatively estimated the intensity of stray light to be
~10–15% relative to the intensity of the evanescent wave at the TIRF
interface [Mattheyses, A. L., and Axelrod D. Direct measurement of the
evanescent field profile produced by objective-based total internal
reflection fluorescence. J Biomed Opt. 2006 Jan-Feb;11(1):014006.]
There are two 2014 papers by Martin Oheim’s group that referenced most
of the literature and analyzed sources of stray light, but did not make
a quantitative comparison. [Brunstein M, Teremetz M, Hérault K, Tourain
C, Oheim M. Eliminating unwanted far-field excitation in objective-type
TIRF. Part I. identifying sources of nonevanescent excitation light.
Biophys J. 2014 Mar 4;106(5):1020-32. Brunstein M, Hérault K, Oheim M.
Eliminating unwanted far-field excitation in objective-type TIRF. Part
II. combined evanescent-wave excitation and supercritical-angle
fluorescence detection improves optical sectioning. Biophys J. 2014 Mar
4;106(5):1044-56.]
Does anybody know additional published or unpublished data with
Quantitative comparison of the stray light with the evanescent wave?
Thank you.
Best regards,
Alexander Asanov, Ph.D.
TIRF Labs
[hidden email]
--
Martin Wessendorf, Ph.D. office: (612) 626-0145
Assoc Prof, Dept Neuroscience lab: (612) 624-2991
University of Minnesota Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009
Minneapolis, MN 55455 e-mail:
[hidden email]