aliquoting 4% formaldehyde

Posted by Cromey, Douglas W - (dcromey) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/aliquoting-4-formaldehyde-tp7585663.html

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I was talking with a student yesterday about their cells which did not look particularly good under the microscope. The discussion eventually came around to fixation. The student told me that he made up some 4% formaldehyde fixative and then froze back (20 deg C) aliquots for later use. This was something he had seem on an online research forum. In my fairly lengthy experience I have never heard of this being done and I am suspicious that this is NOT a good idea. Thoughts?

 

Doug

 

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Douglas W. Cromey, M.S. - Associate Scientific Investigator

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