At my old lab we regularly froze fresh aliquots of EM grade PFA at 16% concentration, -20C for single use in STORM/ PALM (eukaryotes and bacteria) and never had any
problems.
Dr Seamus Holden
University Research Fellow
Centre for Bacterial Cell Biology
Baddiley-Clark Building
Newcastle University
Richardson Road
Newcastle upon Tyne
NE2 4AX, United Kingdom
Phone: +44 (0)191 208 3230
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Cromey, Douglas W - (dcromey)
Sent: 20 September 2016 15:34
To: [hidden email]
Subject: aliquoting 4% formaldehyde
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I was talking with a student yesterday about their cells which did not look particularly good under the microscope. The discussion eventually came around to fixation. The student told me that
he made up some 4% formaldehyde fixative and then froze back (20 deg C) aliquots for later use. This was something he had seem on an online research forum. In my fairly lengthy experience I have never heard of this being done and I am suspicious that this
is NOT a good idea. Thoughts?
Doug
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Douglas W. Cromey, M.S. - Associate Scientific Investigator
Dept. of Cellular & Molecular Medicine, University of Arizona
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